materials human il6 Search Results


gene exp il6 hs00985639 m1  (Thermo Fisher)
99
Thermo Fisher gene exp il6 hs00985639 m1
Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), <t>IL6</t> ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).
Gene Exp Il6 Hs00985639 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pmc08205483-3-14-6?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
gene exp il6 hs00985639 m1 - by Bioz Stars, 2026-07
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gene exp il6 mm01210733 m1  (Thermo Fisher)
93
Thermo Fisher gene exp il6 mm01210733 m1
Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), <t>IL6</t> ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).
Gene Exp Il6 Mm01210733 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/us10933104-191-52-14?v=Thermo+Fisher
Average 93 stars, based on 1 article reviews
gene exp il6 mm01210733 m1 - by Bioz Stars, 2026-07
93/100 stars
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gene exp il6 hs00174131 m1  (Thermo Fisher)
99
Thermo Fisher gene exp il6 hs00174131 m1
Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), <t>IL6</t> ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).
Gene Exp Il6 Hs00174131 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pmc11995296__ACEL___24___e14361___s001-2-266-11?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
gene exp il6 hs00174131 m1 - by Bioz Stars, 2026-07
99/100 stars
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gene exp il6 hs99999032 m1  (Thermo Fisher)
93
Thermo Fisher gene exp il6 hs99999032 m1
Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), <t>IL6</t> ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).
Gene Exp Il6 Hs99999032 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/10__1089_slash_aid__2015__0185-86-32-26?v=Thermo+Fisher
Average 93 stars, based on 1 article reviews
gene exp il6 hs99999032 m1 - by Bioz Stars, 2026-07
93/100 stars
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gene exp mmp14 hs01037003 g1  (Thermo Fisher)
98
Thermo Fisher gene exp mmp14 hs01037003 g1
Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), <t>IL6</t> ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).
Gene Exp Mmp14 Hs01037003 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pmc06949269__41467_2019_14000_MOESM1_ESM-97-10--1?v=Thermo+Fisher
Average 98 stars, based on 1 article reviews
gene exp mmp14 hs01037003 g1 - by Bioz Stars, 2026-07
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gene exp hmga1 hs00852949 g1  (Thermo Fisher)
86
Thermo Fisher gene exp hmga1 hs00852949 g1
Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), <t>IL6</t> ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).
Gene Exp Hmga1 Hs00852949 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pmc05865673__oncotarget___09___14324___s001-19-14-64?v=Thermo+Fisher
Average 86 stars, based on 1 article reviews
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gene exp ctnnb1 hs00355049 m1  (Thermo Fisher)
99
Thermo Fisher gene exp ctnnb1 hs00355049 m1
Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), <t>IL6</t> ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).
Gene Exp Ctnnb1 Hs00355049 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pmc08571098__Table_1-0-2--1?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
gene exp ctnnb1 hs00355049 m1 - by Bioz Stars, 2026-07
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human il-6 gene transgenic mice  (Funakoshi ltd)
90
Funakoshi ltd human il-6 gene transgenic mice
Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), <t>IL6</t> ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).
Human Il 6 Gene Transgenic Mice, supplied by Funakoshi ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pm15942199-383-16-7?v=Funakoshi+ltd
Average 90 stars, based on 1 article reviews
human il-6 gene transgenic mice - by Bioz Stars, 2026-07
90/100 stars
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gene exp il6 mm00446190 m1  (Thermo Fisher)
99
Thermo Fisher gene exp il6 mm00446190 m1
Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), <t>IL6</t> ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).
Gene Exp Il6 Mm00446190 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pm30244972-308-268--1?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
gene exp il6 mm00446190 m1 - by Bioz Stars, 2026-07
99/100 stars
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gene exp tjp1 mm01320638 m1  (Thermo Fisher)
92
Thermo Fisher gene exp tjp1 mm01320638 m1
mRNA expression of TJ proteins, transporters, proinflammatory cytokines and NOS2 in hCMEC/D3 cells exposed to EFV (7,500, 10,000 ng/mL) or vehicle (DMSO) control for 6/24/48 h mRNA expression of <t>TJP1,</t> OCLN, CLDN5, ABCB1, ABCG2, SLC2A1, IL6, IL1β, IL8 and NOS2 in hCMEC/D3 cells exposed to EFV relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping human cyclophilin B gene from n = 4 independent experiments. One-way ANOVA with Bonferroni’s post-hoc test, *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Gene Exp Tjp1 Mm01320638 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pmc10030948-63-14--1?v=Thermo+Fisher
Average 92 stars, based on 1 article reviews
gene exp tjp1 mm01320638 m1 - by Bioz Stars, 2026-07
92/100 stars
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gene exp iltifb mm04203745 mh  (Thermo Fisher)
85
Thermo Fisher gene exp iltifb mm04203745 mh
mRNA expression of TJ proteins, transporters, proinflammatory cytokines and NOS2 in hCMEC/D3 cells exposed to EFV (7,500, 10,000 ng/mL) or vehicle (DMSO) control for 6/24/48 h mRNA expression of <t>TJP1,</t> OCLN, CLDN5, ABCB1, ABCG2, SLC2A1, IL6, IL1β, IL8 and NOS2 in hCMEC/D3 cells exposed to EFV relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping human cyclophilin B gene from n = 4 independent experiments. One-way ANOVA with Bonferroni’s post-hoc test, *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Gene Exp Iltifb Mm04203745 Mh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pmc05504188__Data_Sheet_1-46-39--1?v=Thermo+Fisher
Average 85 stars, based on 1 article reviews
gene exp iltifb mm04203745 mh - by Bioz Stars, 2026-07
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gene exp il6 mm99999064 m1  (Thermo Fisher)
99
Thermo Fisher gene exp il6 mm99999064 m1
mRNA expression of TJ proteins, transporters, proinflammatory cytokines and NOS2 in hCMEC/D3 cells exposed to EFV (7,500, 10,000 ng/mL) or vehicle (DMSO) control for 6/24/48 h mRNA expression of <t>TJP1,</t> OCLN, CLDN5, ABCB1, ABCG2, SLC2A1, IL6, IL1β, IL8 and NOS2 in hCMEC/D3 cells exposed to EFV relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping human cyclophilin B gene from n = 4 independent experiments. One-way ANOVA with Bonferroni’s post-hoc test, *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.
Gene Exp Il6 Mm99999064 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/materials+human+il6/pm39015379-100-36-5?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
gene exp il6 mm99999064 m1 - by Bioz Stars, 2026-07
99/100 stars
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Image Search Results


Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), IL6 ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).

Journal: eLife

Article Title: Polymerization of misfolded Z alpha-1 antitrypsin protein lowers CX3CR1 expression in human PBMCs

doi: 10.7554/eLife.64881

Figure Lengend Snippet: Peripheral blood mononuclear cells (PBMCs) were treated with Z-AAT or lipopolysaccharide (LPS) (used as a positive control) for 18 hr. IL1B ( a ), IL6 ( b ), and TNFA ( c ) mRNA expression relative to HPRT1 housekeeping gene was determined by real-time PCR using Taqman gene expression assays. Data presented as median (IQR) in box and whisker plots, lines represent medians, outliers are defined as data points located outside the whiskers. n = 4 independent experiments. p-Values were calculated by Kruskal-Wallis one-way analysis. Figure 4—figure supplement 2—source data 1. Source files, containing original data for to document IL1B (a), IL6 (b), and TNFA (c) gene expression in peripheral blood mononuclear cells (PBMCs).

Article Snippet: Sequenced-based reagent (human) , IL6 , Thermo Fisher Scientific , Taqman assay 4331182 , Hs00985639_m1.

Techniques: Positive Control, Expressing, Real-time Polymerase Chain Reaction, Gene Expression, Whisker Assay

Primers for gene expression analysis.

Journal: eLife

Article Title: Polymerization of misfolded Z alpha-1 antitrypsin protein lowers CX3CR1 expression in human PBMCs

doi: 10.7554/eLife.64881

Figure Lengend Snippet: Primers for gene expression analysis.

Article Snippet: Sequenced-based reagent (human) , IL6 , Thermo Fisher Scientific , Taqman assay 4331182 , Hs00985639_m1.

Techniques: Gene Expression

Journal: eLife

Article Title: Polymerization of misfolded Z alpha-1 antitrypsin protein lowers CX3CR1 expression in human PBMCs

doi: 10.7554/eLife.64881

Figure Lengend Snippet:

Article Snippet: Sequenced-based reagent (human) , IL6 , Thermo Fisher Scientific , Taqman assay 4331182 , Hs00985639_m1.

Techniques: TaqMan Assay, Clinical Proteomics, Purification, Polymer, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Recombinant, Software

mRNA expression of TJ proteins, transporters, proinflammatory cytokines and NOS2 in hCMEC/D3 cells exposed to EFV (7,500, 10,000 ng/mL) or vehicle (DMSO) control for 6/24/48 h mRNA expression of TJP1, OCLN, CLDN5, ABCB1, ABCG2, SLC2A1, IL6, IL1β, IL8 and NOS2 in hCMEC/D3 cells exposed to EFV relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping human cyclophilin B gene from n = 4 independent experiments. One-way ANOVA with Bonferroni’s post-hoc test, *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Antiretroviral drugs efavirenz, dolutegravir and bictegravir dysregulate blood-brain barrier integrity and function

doi: 10.3389/fphar.2023.1118580

Figure Lengend Snippet: mRNA expression of TJ proteins, transporters, proinflammatory cytokines and NOS2 in hCMEC/D3 cells exposed to EFV (7,500, 10,000 ng/mL) or vehicle (DMSO) control for 6/24/48 h mRNA expression of TJP1, OCLN, CLDN5, ABCB1, ABCG2, SLC2A1, IL6, IL1β, IL8 and NOS2 in hCMEC/D3 cells exposed to EFV relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping human cyclophilin B gene from n = 4 independent experiments. One-way ANOVA with Bonferroni’s post-hoc test, *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Specific human/mouse primer pairs for TJP1/Tjp1 (ZO-1/Zo-1; Hs01551861_m1/Mm01320638_m1), OCLN/Ocln (OCLN/Ocln; Hs00170162_m1/Mm00500912_m1), CLDN5/Cldn5 (CLDN5/Cldn5; Hs00533949_s1/Mm00727012_s1), IL6/Il6 (IL6/Il6; Hs00174131_m1/Mm00446190_m1), IL1β/Il1β (IL1β/Il1β; Hs01555410_m1/Mm00434228_m1), NOS2/Nos2 (iNOS/inos; Hs01075529_m1/Mm00440485_m1), ABCB1/Abcb1a (P-gp/P-gp; Hs00184500_m1/Mm00440761_m1), ABCG2/Abcg2 (BCRP/Bcrp; Hs01053790_m1/Mm00496364_m1), SLC2A1/Slc2a1 (Glut-1/Glut-1; Hs00892681_m1/Mm00441480_m1), and human primer pairs for CXCL8 (IL8; Hs00174103_m1) were designed and validated by Life Technologies for use with TaqMan qPCR chemistry.

Techniques: Expressing, Control

mRNA expression of TJ proteins, transporters, proinflammatory cytokines and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to EFV (7,500, 10,000 ng/mL) and vehicle (DMSO) control for 6 or 24 h and in isolated mouse brain capillaries exposed to EFV (10,000 ng/mL) and vehicle (DMSO) control for 5 h (A) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1, Il6, Il1β and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to EFV relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 4 independent experiments. (B) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1 and Il1β in isolated mouse brain capillaries treated for 5 h with EFV (10,000 ng/mL) relative to vehicle (DMSO) was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 3 independent experiments, where each experiment contained pooled brain tissues from 6 animals per group. One-way ANOVA with Bonferroni’s post-hoc test (A) , unpaired two-tailed Student’s t -test (B) : *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Antiretroviral drugs efavirenz, dolutegravir and bictegravir dysregulate blood-brain barrier integrity and function

doi: 10.3389/fphar.2023.1118580

Figure Lengend Snippet: mRNA expression of TJ proteins, transporters, proinflammatory cytokines and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to EFV (7,500, 10,000 ng/mL) and vehicle (DMSO) control for 6 or 24 h and in isolated mouse brain capillaries exposed to EFV (10,000 ng/mL) and vehicle (DMSO) control for 5 h (A) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1, Il6, Il1β and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to EFV relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 4 independent experiments. (B) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1 and Il1β in isolated mouse brain capillaries treated for 5 h with EFV (10,000 ng/mL) relative to vehicle (DMSO) was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 3 independent experiments, where each experiment contained pooled brain tissues from 6 animals per group. One-way ANOVA with Bonferroni’s post-hoc test (A) , unpaired two-tailed Student’s t -test (B) : *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Specific human/mouse primer pairs for TJP1/Tjp1 (ZO-1/Zo-1; Hs01551861_m1/Mm01320638_m1), OCLN/Ocln (OCLN/Ocln; Hs00170162_m1/Mm00500912_m1), CLDN5/Cldn5 (CLDN5/Cldn5; Hs00533949_s1/Mm00727012_s1), IL6/Il6 (IL6/Il6; Hs00174131_m1/Mm00446190_m1), IL1β/Il1β (IL1β/Il1β; Hs01555410_m1/Mm00434228_m1), NOS2/Nos2 (iNOS/inos; Hs01075529_m1/Mm00440485_m1), ABCB1/Abcb1a (P-gp/P-gp; Hs00184500_m1/Mm00440761_m1), ABCG2/Abcg2 (BCRP/Bcrp; Hs01053790_m1/Mm00496364_m1), SLC2A1/Slc2a1 (Glut-1/Glut-1; Hs00892681_m1/Mm00441480_m1), and human primer pairs for CXCL8 (IL8; Hs00174103_m1) were designed and validated by Life Technologies for use with TaqMan qPCR chemistry.

Techniques: Expressing, Control, Isolation, Two Tailed Test

mRNA expression of TJ proteins, transporters, proinflammatory cytokines and NOS2 in hCMEC/D3 cells exposed to DTG (2,000, 5,000 ng/mL) or vehicle (DMSO) control for 6/24/48 h mRNA expression of TJP1, OCLN, CLDN5, ABCB1, ABCG2, SLC2A1, IL6, IL1β, IL8 and NOS2 in hCMEC/D3 cells exposed to DTG relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping human cyclophilin B gene from n = 4 independent experiments. One-way ANOVA with Bonferroni’s post-hoc test, *, p < 0.05; ***, p < 0.001; ****, p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Antiretroviral drugs efavirenz, dolutegravir and bictegravir dysregulate blood-brain barrier integrity and function

doi: 10.3389/fphar.2023.1118580

Figure Lengend Snippet: mRNA expression of TJ proteins, transporters, proinflammatory cytokines and NOS2 in hCMEC/D3 cells exposed to DTG (2,000, 5,000 ng/mL) or vehicle (DMSO) control for 6/24/48 h mRNA expression of TJP1, OCLN, CLDN5, ABCB1, ABCG2, SLC2A1, IL6, IL1β, IL8 and NOS2 in hCMEC/D3 cells exposed to DTG relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping human cyclophilin B gene from n = 4 independent experiments. One-way ANOVA with Bonferroni’s post-hoc test, *, p < 0.05; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Specific human/mouse primer pairs for TJP1/Tjp1 (ZO-1/Zo-1; Hs01551861_m1/Mm01320638_m1), OCLN/Ocln (OCLN/Ocln; Hs00170162_m1/Mm00500912_m1), CLDN5/Cldn5 (CLDN5/Cldn5; Hs00533949_s1/Mm00727012_s1), IL6/Il6 (IL6/Il6; Hs00174131_m1/Mm00446190_m1), IL1β/Il1β (IL1β/Il1β; Hs01555410_m1/Mm00434228_m1), NOS2/Nos2 (iNOS/inos; Hs01075529_m1/Mm00440485_m1), ABCB1/Abcb1a (P-gp/P-gp; Hs00184500_m1/Mm00440761_m1), ABCG2/Abcg2 (BCRP/Bcrp; Hs01053790_m1/Mm00496364_m1), SLC2A1/Slc2a1 (Glut-1/Glut-1; Hs00892681_m1/Mm00441480_m1), and human primer pairs for CXCL8 (IL8; Hs00174103_m1) were designed and validated by Life Technologies for use with TaqMan qPCR chemistry.

Techniques: Expressing, Control

mRNA expression of TJ proteins, transporters, proinflammatory cytokines and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to DTG (2000, 5,000 ng/mL) and vehicle (DMSO) control for 6 or 24 h and in isolated mouse brain capillaries exposed to DTG (5,000 ng/mL) and vehicle (DMSO) control for 5 h (A) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1, Il6, Il1β and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to DTG relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 4 independent experiments. (B) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1 and Il1β in isolated mouse brain capillaries treated for 5 h with DTG (5,000 ng/mL) relative to vehicle (DMSO) was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 3 independent experiments, where each experiment contained pooled brain tissues from 6 animals per group. One-way ANOVA with Bonferroni’s post-hoc test (A) , unpaired two-tailed Student’s t -test (B) : *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Antiretroviral drugs efavirenz, dolutegravir and bictegravir dysregulate blood-brain barrier integrity and function

doi: 10.3389/fphar.2023.1118580

Figure Lengend Snippet: mRNA expression of TJ proteins, transporters, proinflammatory cytokines and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to DTG (2000, 5,000 ng/mL) and vehicle (DMSO) control for 6 or 24 h and in isolated mouse brain capillaries exposed to DTG (5,000 ng/mL) and vehicle (DMSO) control for 5 h (A) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1, Il6, Il1β and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to DTG relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 4 independent experiments. (B) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1 and Il1β in isolated mouse brain capillaries treated for 5 h with DTG (5,000 ng/mL) relative to vehicle (DMSO) was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 3 independent experiments, where each experiment contained pooled brain tissues from 6 animals per group. One-way ANOVA with Bonferroni’s post-hoc test (A) , unpaired two-tailed Student’s t -test (B) : *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Specific human/mouse primer pairs for TJP1/Tjp1 (ZO-1/Zo-1; Hs01551861_m1/Mm01320638_m1), OCLN/Ocln (OCLN/Ocln; Hs00170162_m1/Mm00500912_m1), CLDN5/Cldn5 (CLDN5/Cldn5; Hs00533949_s1/Mm00727012_s1), IL6/Il6 (IL6/Il6; Hs00174131_m1/Mm00446190_m1), IL1β/Il1β (IL1β/Il1β; Hs01555410_m1/Mm00434228_m1), NOS2/Nos2 (iNOS/inos; Hs01075529_m1/Mm00440485_m1), ABCB1/Abcb1a (P-gp/P-gp; Hs00184500_m1/Mm00440761_m1), ABCG2/Abcg2 (BCRP/Bcrp; Hs01053790_m1/Mm00496364_m1), SLC2A1/Slc2a1 (Glut-1/Glut-1; Hs00892681_m1/Mm00441480_m1), and human primer pairs for CXCL8 (IL8; Hs00174103_m1) were designed and validated by Life Technologies for use with TaqMan qPCR chemistry.

Techniques: Expressing, Control, Isolation, Two Tailed Test

mRNA expression of TJ proteins, transporters, proinflammatory cytokines and NOS2 in hCMEC/D3 cells exposed to BTG (3,000, 6,000 ng/mL) or vehicle (DMSO) control for 6 or 24 h mRNA expression of TJP1, OCLN, CLDN5, ABCB1, ABCG2, SLC2A1, IL6, IL1β, IL8 and NOS2 in hCMEC/D3 cells exposed to BTG relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping human cyclophilin B gene from n = 4 independent experiments. One-way ANOVA with Bonferroni’s post-hoc test, *, p < 0.05; **, p < 0.01.

Journal: Frontiers in Pharmacology

Article Title: Antiretroviral drugs efavirenz, dolutegravir and bictegravir dysregulate blood-brain barrier integrity and function

doi: 10.3389/fphar.2023.1118580

Figure Lengend Snippet: mRNA expression of TJ proteins, transporters, proinflammatory cytokines and NOS2 in hCMEC/D3 cells exposed to BTG (3,000, 6,000 ng/mL) or vehicle (DMSO) control for 6 or 24 h mRNA expression of TJP1, OCLN, CLDN5, ABCB1, ABCG2, SLC2A1, IL6, IL1β, IL8 and NOS2 in hCMEC/D3 cells exposed to BTG relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping human cyclophilin B gene from n = 4 independent experiments. One-way ANOVA with Bonferroni’s post-hoc test, *, p < 0.05; **, p < 0.01.

Article Snippet: Specific human/mouse primer pairs for TJP1/Tjp1 (ZO-1/Zo-1; Hs01551861_m1/Mm01320638_m1), OCLN/Ocln (OCLN/Ocln; Hs00170162_m1/Mm00500912_m1), CLDN5/Cldn5 (CLDN5/Cldn5; Hs00533949_s1/Mm00727012_s1), IL6/Il6 (IL6/Il6; Hs00174131_m1/Mm00446190_m1), IL1β/Il1β (IL1β/Il1β; Hs01555410_m1/Mm00434228_m1), NOS2/Nos2 (iNOS/inos; Hs01075529_m1/Mm00440485_m1), ABCB1/Abcb1a (P-gp/P-gp; Hs00184500_m1/Mm00440761_m1), ABCG2/Abcg2 (BCRP/Bcrp; Hs01053790_m1/Mm00496364_m1), SLC2A1/Slc2a1 (Glut-1/Glut-1; Hs00892681_m1/Mm00441480_m1), and human primer pairs for CXCL8 (IL8; Hs00174103_m1) were designed and validated by Life Technologies for use with TaqMan qPCR chemistry.

Techniques: Expressing, Control

mRNA expression of TJ proteins, transporters, proinflammatory cytokines and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to BTG (3,000, 6,000 ng/mL) and vehicle (DMSO) control for 6 or 24 h and in isolated mouse brain capillaries exposed to BTG (6,000 ng/mL) and vehicle (DMSO) control for 5 h (A) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1, Il6, Il1β and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to BTG relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 4 independent experiments. (B) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1 and Il1β in isolated mouse brain capillaries treated for 5 h with BTG (6,000 ng/mL) relative to vehicle (DMSO) was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 3 independent experiments, where each experiment contained pooled brain tissues from 6 animals per group. One-way ANOVA with Bonferroni’s post-hoc test (A) , unpaired two-tailed Student’s t -test (B) : *, p < 0.05; ***, p < 0.001.

Journal: Frontiers in Pharmacology

Article Title: Antiretroviral drugs efavirenz, dolutegravir and bictegravir dysregulate blood-brain barrier integrity and function

doi: 10.3389/fphar.2023.1118580

Figure Lengend Snippet: mRNA expression of TJ proteins, transporters, proinflammatory cytokines and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to BTG (3,000, 6,000 ng/mL) and vehicle (DMSO) control for 6 or 24 h and in isolated mouse brain capillaries exposed to BTG (6,000 ng/mL) and vehicle (DMSO) control for 5 h (A) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1, Il6, Il1β and Nos2 in primary cultures of mouse brain microvascular endothelial cells exposed to BTG relative to vehicle (DMSO) control was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 4 independent experiments. (B) mRNA expression of Tjp1, Ocln, Cldn5, Abcb1a, Abcg2, Slc2a1 and Il1β in isolated mouse brain capillaries treated for 5 h with BTG (6,000 ng/mL) relative to vehicle (DMSO) was assessed by qPCR. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 3 independent experiments, where each experiment contained pooled brain tissues from 6 animals per group. One-way ANOVA with Bonferroni’s post-hoc test (A) , unpaired two-tailed Student’s t -test (B) : *, p < 0.05; ***, p < 0.001.

Article Snippet: Specific human/mouse primer pairs for TJP1/Tjp1 (ZO-1/Zo-1; Hs01551861_m1/Mm01320638_m1), OCLN/Ocln (OCLN/Ocln; Hs00170162_m1/Mm00500912_m1), CLDN5/Cldn5 (CLDN5/Cldn5; Hs00533949_s1/Mm00727012_s1), IL6/Il6 (IL6/Il6; Hs00174131_m1/Mm00446190_m1), IL1β/Il1β (IL1β/Il1β; Hs01555410_m1/Mm00434228_m1), NOS2/Nos2 (iNOS/inos; Hs01075529_m1/Mm00440485_m1), ABCB1/Abcb1a (P-gp/P-gp; Hs00184500_m1/Mm00440761_m1), ABCG2/Abcg2 (BCRP/Bcrp; Hs01053790_m1/Mm00496364_m1), SLC2A1/Slc2a1 (Glut-1/Glut-1; Hs00892681_m1/Mm00441480_m1), and human primer pairs for CXCL8 (IL8; Hs00174103_m1) were designed and validated by Life Technologies for use with TaqMan qPCR chemistry.

Techniques: Expressing, Control, Isolation, Two Tailed Test

mRNA expression of TJ proteins, membrane associated transporters and proinflammatory cytokine in mouse brain capillaries. The mRNA expression of Tjp1, Ocln, Cldn5 , Abcb1a, Abcg2, Slc2a1 or Il1β genes was assessed by qPCR in brain capillaries isolated from mice treated with DTG (5 mg/kg/day), BTG (5 mg/kg/day) or vehicle (regular PB pellet) for 14 days. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 6 animals per group. One-way ANOVA with Bonferroni’s post-hoc test: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Antiretroviral drugs efavirenz, dolutegravir and bictegravir dysregulate blood-brain barrier integrity and function

doi: 10.3389/fphar.2023.1118580

Figure Lengend Snippet: mRNA expression of TJ proteins, membrane associated transporters and proinflammatory cytokine in mouse brain capillaries. The mRNA expression of Tjp1, Ocln, Cldn5 , Abcb1a, Abcg2, Slc2a1 or Il1β genes was assessed by qPCR in brain capillaries isolated from mice treated with DTG (5 mg/kg/day), BTG (5 mg/kg/day) or vehicle (regular PB pellet) for 14 days. Results are presented as mean relative mRNA expression ±SEM normalized to the housekeeping mouse cyclophilin B gene from n = 6 animals per group. One-way ANOVA with Bonferroni’s post-hoc test: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Specific human/mouse primer pairs for TJP1/Tjp1 (ZO-1/Zo-1; Hs01551861_m1/Mm01320638_m1), OCLN/Ocln (OCLN/Ocln; Hs00170162_m1/Mm00500912_m1), CLDN5/Cldn5 (CLDN5/Cldn5; Hs00533949_s1/Mm00727012_s1), IL6/Il6 (IL6/Il6; Hs00174131_m1/Mm00446190_m1), IL1β/Il1β (IL1β/Il1β; Hs01555410_m1/Mm00434228_m1), NOS2/Nos2 (iNOS/inos; Hs01075529_m1/Mm00440485_m1), ABCB1/Abcb1a (P-gp/P-gp; Hs00184500_m1/Mm00440761_m1), ABCG2/Abcg2 (BCRP/Bcrp; Hs01053790_m1/Mm00496364_m1), SLC2A1/Slc2a1 (Glut-1/Glut-1; Hs00892681_m1/Mm00441480_m1), and human primer pairs for CXCL8 (IL8; Hs00174103_m1) were designed and validated by Life Technologies for use with TaqMan qPCR chemistry.

Techniques: Expressing, Membrane, Isolation